w66利最给力的老牌平台

教育经历

2002-2006  南开大学生命科学学院，学士

2006-2012  美国科罗拉多大学医学中心 (University of Colorado Anschutz Medical Campus)，博士

工作经历与任职

2012-2015  美国哈佛大学医学院 (Harvard Medical School)，博士后

2015-2018  美国CRISPR基因治疗公司 (CRISPR Therapeutics)，资深研究员

2018-    w66利最给力的老牌平台，教授

社会兼职

2021-  CELL Insight期刊    副主编

获奖情况

2019  获湖北省科技创新bairen计划

2012  获杰出博士论文奖    科罗拉多大学医学中心

2009  获第24届杰出科研奖   科罗拉多大学医学中心

研究领域

通过CRISPR技术直接修复胞内致病突变，或增强免疫细胞抗肿瘤功能来治疗癌症，从而将 CRISPR 技术转化为细胞疗法。 本课题组的目标是设计并构建完整的细胞基因编辑工具，并安全有效地将其递送至原代细胞中实现精准治疗。主要包括：

  1. 新型CRISPR/Cas9工具的构建与优化；

  2. 优化用于细胞治疗的大分子非病毒递送；

  3. 探究CRISPR/Cas9 介导的细胞疗法，包括免疫疗法和对遗传疾病的治疗。

代表性论文

1. Hong-Xia Zhang^#, Caixiang Zhang^#, Shuhan Lu, Xiaohan Tong, Kun Zhang, Hao Yin*, Ying Zhang*. Cas12a-based one-pot SNP detection with high accuracy. Cell Insight, (2023): 100080. doi.org/10.1016/j.cellin.2023.100080

2. Houyuan Qiu, Ruijin Ji, Ying Zhang*. Current advances of CRISPR-Cas technology in cell therapy. Cell Insight, (2022): 100067 doi.org/10.1016/j.cellin.2022.100067

3. Ya-jing Shi#, Min Duan#, Junmei Ding#, Fan-qi Wang, Lili Bi, Caixiang Zhang, Yi-zhou Zhang, Jun-yi Duan, An-hui Huang, Xin-lin Lei, Hao Yin, Ying Zhang*. DNA topology regulates PAM-Cas9 interaction and DNA unwinding to enable near PAMless cleavage by thermophilic Cas9. Molecular Cell, Nov 3; 82(21): 4160-4175, 2022

4. Shuhan Lu#, Xiaohan Tong#, Yang Han#, Kun Zhang, Yizhou Zhang, Qiubing Chen, Junyi Duan, Xinlin Lei, Muhan Huang, Yang Qiu, Ding-Yu Zhang, Xi Zhou, Ying Zhang* and Hao Yin*. Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a. Nature Biomedical Engineering, in press, 2022

5. Jinlin Wang#, Zhou He#, Guoquan Wang#, Ruiwen Zhang#, Junyi Duan, Pan Gao, Xinlin Lei, Houyuan Qiu, Chuanping Zhang, Ying Zhang and Hao Yin*. Efficient targeted insertion of large DNA fragments without DNA donors. Nature Methods, 2022, Feb 28

5. Wang, Lijie#; Xue, Wei#; Zhang, Hongxia#; Gao, Runze#; Qiu, Houyuan#; Wei, Jia; Zhou, Lina; Lei, Yun-Ni; Wu, Xiaocheng; Li, Xiao; Liu, Chengfang; Wu, Jing; Chen, Qiubing; Ma, Hanhui; Huang, Xingxu; Cai, Cheguo; Zhang, Ying; Yang, Bei*; Yin, Hao*; Yang, Li*; Chen, Jia*; Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations. Nature Cell Biology, 2021, 23(5): 552–563.

6. Chen Q, Zhang Y*, Yin H*. Recent advances in chemical modifications of guide RNA, mRNA and donor template for CRISPR-mediated genome editing. Advanced Drug Delivery Reviews. 2020 Oct 27;S0169-409X(20)30159-9.

7. Shuhan Lu, Ying Zhang*, Hao Yin*. Chimeric DNA-RNA Guide RNA Designs. Methods Mol Biol. 2021;2162:79-85.

8. Lu, Shuhan; Li, Fang; Chen, Qiubing; Wu, Jing; Duan, Junyi; Lei, Xinlin; Zhang, Ying; Zhao, Dongming; Bu, Zhigao*; Yin, Hao*; Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics, Cell Discovery, 2020, 6(1)：0-18.

9. Zhang Y*, Yin H*. Genome Editing for Genetic Lung diseases. Pharmaceutical Inhalation Aerosol Technology, Third Edition. 2019

10. Zhang HX, Zhang Y*, Yin H*. Genome Editing with mRNA Encoding ZFN, TALEN, and Cas9. Molecular Therapy, 2019. Apr 10;27(4):735-746.

11. Liu Y, Lin H, Liu Z, Ouyang H, Signer R, Huang S, Chen S, Wu F, Zhang Y, Lin D, Zhu J, Patel S, Qiu A, Li G, Cai H, Cao G, Zhu J, Chen D, Shang F, Liu X, Luo L, Granet D, Li X, Morrison S, Zhang K. Lens regeneration using endogenous stem cells with gain of visual function. Nature. 2016 Mar 17;531(7594):323-8.

12. Zhang Y, Fan J, Ho J, Hu T, Kneeland S, Fan X, Sellarole M, Vries W, Lu W, Lang R, John S, Maas R. Crim1 regulates integrin signaling in murine lens development. Development. 2016 Jan 15;143(2):356-66.

13. Chen J, Zhang Y, Wilde J, Hansen K, Lai F, Niswander L. Microcephaly disease gene Wdr62 regulates mitotic progression of embryonic neural stem cells and brain size. Nature Communications. 2014 May 30;5:3885

14. Zhang Y, Niswander L. Zic2 is required for enteric nervous system development and neurite outgrowth: a mouse model of enteric hyperplasia and dysplasia. Neurogastroenterol Motil. 2013 Jun;25(6):538-41.

15. Zhang, Y. and Niswander, L. Phactr4: a new integrin modulator required for directional migration of enteric neural crest cells. Cell Adh Migr. 2012 Sep-Oct;6(5):419-23.

16. Zhang Y#, Kim TH#, Niswander L. Phactr4 regulates directional migration of Enteric Neural Crest through PP1, integrin signaling and cofilin activity. Genes & Development. 2012 Jan 1; 26(1):69-81. (Evaluated in Faculty of 1000 and highlighted at Genes Dev. 2012 Jan 1; 26(1):1-5. Nature Cell Biology 14, 130 (2012) doi:10.1038/ncb2436 Sci. Signal., 10 January 2012, Vol. 5, Issue 206, p. ec12)

17. Marean A, Graf A, Zhang Y, Niswander L. Folic acid supplementation can adversely affect murine neural tube closure and embryonic survival. Hum Mol Genet. 2011 Sep 15;20(18):3678-83.

18. Yang J, Liang L, Zhang Y, Li J, Zhang L, Ye F, Gan Z, Zhang KQ. Purification and cloning of a novel serine protease from the nematode-trapping fungus Dactylellinavarietas and its potential roles in infection against nematodes. Appl. Microbiol. Biotechnol. 2007 Jun;75(3):557-65.

专利 

1. Patent number: PCT/CN2023/072703. COMPOSITIONS AND METHODS FOR TREATING ACUTE MYELOID LEUKEMIA. Ying Zhang, Guohua Cao, Ruijin Ji. Filing date January 17th, 2023 （申请）

2. Patent number: PCT/CN2021/107047. Compositions and Methods for Effective Delivery of Polynucleotides to Nuclei. Ying Zhang, Hao Yin, Chuanping Zhang. Filing date July 19th, 2021 （申请）

3. Patent number: PCT/CN2022/077097. Editing of Double-Stranded DNA with Relaxed PAM Requirement. Ying Zhang, Ya-jing Shi. Filing date Feb 21th, 2022 （申请）

4. Patent number: PCT/CN2022/070850. Compositions and Methods for Instant Nucleic Acid Detection.  Hao Yin, Shuhan Lu, Ying Zhang, Xiaohan Tong, Kun Zhang, Xi Zhou, Dingyu Zhang. Filing date Jan 8th, 2021 （申请）

5. Patent number: WO2017141109 A1. Materials and methods for treatment of severe combined immunodeficiency (SCID) or omenn syndrome. Ying ZHANG, Chad Albert COWAN, Ante Sven LUNDBERG, Gregory Joseph COST. Filing date Feb 17, 2017. （申请）

6. Patent number: 200410040796.X.  The preparation and the application of an antifungal reagent  
    一种防治植物寄生线虫的生防制剂，张楹等，专利号：ZL  03135134.4，授权时间2005年

7. Materials and methods for treatment of severe combined immunodeficiency (SCID) or omenn syndrome. Ying ZHANG, Chad Albert COWAN, Ante Sven LUNDBERG, Gregory Joseph COST. PCT/IB2017/000200

8. The preparation and the application of an antifungal reagent. Ying ZHANG. 200410040796.X.

发表著作

Book Chapter "Genome Editing for Genetic Lung Diseases" Pharmaceutical Inhalation Aerosol Technology Third Edition.

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